prolong ® gold antifade mountant with dna stain 4’,6-diamidino-2-phenylindole, dihydrochloride (dapi) (Thermo Fisher)
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Prolong ® Gold Antifade Mountant With Dna Stain 4’,6 Diamidino 2 Phenylindole, Dihydrochloride (Dapi), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prolong+gold+antifade+mountant+with+dna+stain+dapi/prolong+++gold+antifade+mountant+with+dna+stain+4%E2%80%99+6+diamidino+2+phenylindole++dihydrochloride++dapi+/pmc12031635-88-70-73
Average 90 stars, based on 1 article reviews
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1) Product Images from "The Virus Entry Pathway Determines Sensitivity to the Antiviral Peptide TAT-I24"
Article Title: The Virus Entry Pathway Determines Sensitivity to the Antiviral Peptide TAT-I24
Journal: Viruses
doi: 10.3390/v17040458
Figure Legend Snippet: Effect of peptides on dsRNA levels in infected Vero E6 cells. ( A ) Cells were infected with Wuhan variant (500 pfu/well) or ( B ) Delta variant (500 pfu/well) for 2 h and 24 h in absence (untreated) and presence of TAT-I24 (10 µM) or TAT (10 µM), followed by staining for dsRNA (FITC/green); nuclei were stained with DAPI (blue), and one representative section is shown. Scale bars indicate 50 µm. ( C ) Quantification of dsRNA positive signals (FITC/green) from three randomly chosen sections normalized against DAPI (blue) signals and expressed as % of control; mean + SD from dsRNA relative to untreated controls are shown. Significant differences were detected using one-way ANOVA (Kruskal–Wallis multiple comparison of means); p -value: p ≤ 0.05 (*).
Techniques Used: Infection, Variant Assay, Staining, Control, Comparison
Figure Legend Snippet: Localization of TAT-I24 in endosomal compartments. ( A ) Localization of Rho-TAT-I24 (red) in COS-7 cells after 15, 90, and 180 min incubation or ( B ) in Vero E6 cells after 30 and 120 min incubation. ( C ) Localization of Rho-TAT-I24 after 120 min either alone or in presence of 30 µM of CQ. ( D ) Staining of COS-7 cells for LAMP1 (green) after 30 min of incubation with Rho-TAT-I24 (red). Arrows indicate co-localization of Rho-TAT-I24 and LAMP1. ( E ) Overexpression of GFP-Rab5 (green) and lack of co-localization with Rho-TAT-I24 (red), or ( F ) overexpression of GFP-Rab14 (green) and incubation with Rho-TAT-I24 (red). Arrows indicate co-localization of GFP-Rab14 and Rho-TAT-I24 in enlarged vesicles. Nuclei are stained with DAPI (blue). Scale bars indicate 40 µm. ( G ) Co-localization of GFP-Rab5 or GFP-Rab14 with Rho-TAT-I24, expressed as percent (%) of total GFP signal, was determined from four areas of two independent experiments using color threshold tool in ImageJ.
Techniques Used: Incubation, Staining, Over Expression
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